Inter-laboratory validation of the serial dilution culture—most probable number method for enumerating viable phytoplankton
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چکیده
منابع مشابه
Reliability of the most-probable-number technique for enumerating rhizobia in tropical soils.
We used six rhizobium-legume systems to test the reliability of the most-probable-number (MPN) technique for enumerating rhizobia introduced into 14 sites representing four soil orders. The range-of-transition values (the number of dilution steps between the first not-entirely-positive and the last not-entirely-negative growth units) were compared for each species and for each soil. The probabi...
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Nine different receptacles were tested with the MPN method to determine which receptacle was most reliable and economical for MPN counts. Results showed that 96 well PCR plate were the best vessels for this type of analysis and facilitated the isolation of viable Methylotrophs.
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A single-step most-probable-number method for enumerating fecal coliforms in sewage treatment plant effluents is described. The method requires the use of only one lactose-based medium and a single incubation temperature of 44.5 degrees C, and it can be completed in 18 h or less, as compared with up to 72 h for the standard most-probable-number method. The appearance of growth is the sole crite...
متن کاملThe Most Probable Number Method and Its Uses in Enumeration, Qualification, and Validation
"Microbiology Topics" discusses various topics in microbiology of practical use in validation and com pliance. We intend this column to be a useful resource for daily work applications. Reader comments, questions, and suggestions are needed to help us fulfill our objective for this col umn. Please send your comments and suggestions to column coordinator Scott Sutton at scott.sutton@ microbiol...
متن کاملInter-laboratory validation of bioaccessibility testing for metals.
Bioelution assays are fast, simple alternatives to in vivo testing. In this study, the intra- and inter-laboratory variability in bioaccessibility data generated by bioelution tests were evaluated in synthetic fluids relevant to oral, inhalation, and dermal exposure. Using one defined protocol, five laboratories measured metal release from cobalt oxide, cobalt powder, copper concentrate, Incone...
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ژورنال
عنوان ژورنال: Journal of Applied Phycology
سال: 2018
ISSN: 0921-8971,1573-5176
DOI: 10.1007/s10811-018-1541-z